January 1992
Volume 33, Issue 1
Free
Articles  |   January 1992
Immunocytochemical localization of cyclooxygenase in the rat lens.
Author Affiliations
  • D R Zheng
    Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark.
  • S C Fu
    Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark.
  • T W Lysz
    Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark.
  • C C Leung
    Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark.
Investigative Ophthalmology & Visual Science January 1992, Vol.33, 178-183. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      D R Zheng, S C Fu, T W Lysz, C C Leung; Immunocytochemical localization of cyclooxygenase in the rat lens.. Invest. Ophthalmol. Vis. Sci. 1992;33(1):178-183.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
This content is PDF only. Please click on the PDF icon to access.
Abstract

The localization of rat lens fatty acid cyclooxygenase (prostaglandin synthase) was studied using indirect immunofluorescent and indirect streptavidin-biotin immunoperoxidase staining techniques. Both methods employed monoclonal and polyclonal antibodies directed against fatty acid cyclooxygenase, the key enzyme in the conversion of arachidonic acid to prostaglandins. The immunocytochemical studies demonstrate that (1) fatty acid cyclooxygenase is present in the rat lens epithelial cell layer; (2) the enzyme appears predominantly in the cytoplasm; (3) there is an apparent higher concentration of the enzyme in the region designated as germinative and transitional zones, and meridional rows; and (4) the enzyme appears to be absent in the lens capsule and in the nucleus of the lens. The presence of the cyclooxygenase enzyme in the lens epithelium, especially the relative intense staining in the epithelial mitogenic region, suggests that oxygenation of polyunsaturated fatty acids by the fatty acid cyclooxygenase may have an important role in cellular differentiation.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×