March 1991
Volume 32, Issue 3
Articles  |   March 1991
The hydration of actin.
Author Affiliations
  • S Kakar
    Chemistry Department, Adelphi University, Garden City, NY 11530.
  • F A Bettelheim
    Chemistry Department, Adelphi University, Garden City, NY 11530.
Investigative Ophthalmology & Visual Science March 1991, Vol.32, 562-566. doi:
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      S Kakar, F A Bettelheim; The hydration of actin.. Invest. Ophthalmol. Vis. Sci. 1991;32(3):562-566.

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      © ARVO (1962-2015); The Authors (2016-present)

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The hydration of actin was studied by differential scanning calorimetry between -30 degrees C and 30 degrees C and by thermogravimetric analysis. The differential scanning calorimetry provided the freezable water content of G- and F-actin as a function of concentration, and the thermogravimetric analysis measured the total water content. The difference between the two yielded the nonfreezable water content (bound water) as a function of concentration. The nonfreezable water content of G-actin was higher than the F-actin over the whole concentration range from 1-40% actin.


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