August 1993
Volume 34, Issue 9
Free
Articles  |   August 1993
Modulation of major histocompatibility complex class 1 genes in human retinoblastoma cells by interferons.
Author Affiliations
  • S Barez
    Immunology and Virology Section, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
  • D T Boumpas
    Immunology and Virology Section, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
  • C M Percopo
    Immunology and Virology Section, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
  • E D Anastassiou
    Immunology and Virology Section, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
  • J J Hooks
    Immunology and Virology Section, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
  • B Detrick
    Immunology and Virology Section, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Investigative Ophthalmology & Visual Science August 1993, Vol.34, 2613-2621. doi:
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      S Barez, D T Boumpas, C M Percopo, E D Anastassiou, J J Hooks, B Detrick; Modulation of major histocompatibility complex class 1 genes in human retinoblastoma cells by interferons.. Invest. Ophthalmol. Vis. Sci. 1993;34(9):2613-2621.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To examine the mechanism(s) of interferon (IFN) induced expression of major histocompatibility complex (MHC) class 1 molecules on the human retinoblastoma cell line, Y-79. METHODS: Y-79 cells were incubated in the presence of IFN-alpha, -beta, and -gamma. Y-79 cell expression of MHC class 1 molecules was measured by flow cytometric analysis. HLA-B7 and oncogene transcription were evaluated by Northern blot analysis and nuclear runoff transcription assays. RESULTS: IFN-gamma increased MHC-class 1 antigen expression and induced a fivefold increase in its transcription rate. Posttranscriptionally, IFN-beta and -gamma increased steady state messenger RNA for the HLA-B7 gene. These effects were not associated with down regulation of N-myc oncogene nuclear transcription. Moreover, dexamethasone did not affect the IFN-gamma induced expression of MHC-class 1 molecules. CONCLUSIONS: Both transcriptional and posttranscriptional mechanisms are implicated in the modulation of class 1 molecule expression by IFN. In addition, this modulation is not associated with down regulation of N-myc oncogene expression. Spontaneous or IFN-gamma induced MHC class 1 antigen expression in retinoblastoma Y-79 cells is resistant to glucocorticoid hormones.

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