December 1993
Volume 34, Issue 13
Free
Articles  |   December 1993
Alternatively spliced fibronectin molecules in the wounded cornea: analysis by PCR.
Author Affiliations
  • X Cai
    Rhoads Molecular Immunology Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
  • C S Foster
    Rhoads Molecular Immunology Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
  • J J Liu
    Rhoads Molecular Immunology Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
  • A E Kupferman
    Rhoads Molecular Immunology Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
  • M Filipec
    Rhoads Molecular Immunology Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
  • R B Colvin
    Rhoads Molecular Immunology Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
  • S J Lee
    Rhoads Molecular Immunology Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston 02114.
Investigative Ophthalmology & Visual Science December 1993, Vol.34, 3585-3592. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      X Cai, C S Foster, J J Liu, A E Kupferman, M Filipec, R B Colvin, S J Lee; Alternatively spliced fibronectin molecules in the wounded cornea: analysis by PCR.. Invest. Ophthalmol. Vis. Sci. 1993;34(13):3585-3592.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
This content is PDF only. Please click on the PDF icon to access.
Abstract

PURPOSE: To determine whether certain fibronectin isoforms participate in corneal epithelial wound healing, the authors used the polymerase chain reaction to detect different splicing patterns of the EIIIA segment of fibronectin mRNA in epithelial scrape-wounded cornea of rats. METHODS: Specific fibronectin cDNA sequences synthesized from rat cornea with total RNA were amplified with various sets of synthetic oligonucleotide primers. RESULTS: The authors detected both the EIIIA+ and EIIIA- fibronectin mRNA isoforms during corneal wound healing. The kinetics of corneal expression of both total fibronectin mRNA and the EIIIA- fibronectin mRNA isoform was polyphasic; an initial decrease was followed by an increase at 45 minutes, a second increase at 2 hours, and a third increase at 4 days after wounding. EIIIA+ fibronectin mRNA, not found in normal cornea, also was detected during healing. CONCLUSIONS: The expression of total fibronectin mRNA and both the EIIIA+ and EIIIA- fibronectin mRNA is upregulated during corneal epithelial wound healing. The expression of EIIIA+ fibronectin mRNA during wound healing, a fibronectin isoform that was highly expressed in embryonic tissue, suggests that this fibronectin isoform is involved functionally in corneal wound healing.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×