April 1992
Volume 33, Issue 5
Free
Articles  |   April 1992
An ocular renin-angiotensin system. Immunohistochemistry of angiotensinogen.
Author Affiliations
  • S J Sramek
    Department of Ophthalmology, University of Wisconsin Medical School, Madison.
  • I H Wallow
    Department of Ophthalmology, University of Wisconsin Medical School, Madison.
  • D A Tewksbury
    Department of Ophthalmology, University of Wisconsin Medical School, Madison.
  • C R Brandt
    Department of Ophthalmology, University of Wisconsin Medical School, Madison.
  • G L Poulsen
    Department of Ophthalmology, University of Wisconsin Medical School, Madison.
Investigative Ophthalmology & Visual Science April 1992, Vol.33, 1627-1632. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      S J Sramek, I H Wallow, D A Tewksbury, C R Brandt, G L Poulsen; An ocular renin-angiotensin system. Immunohistochemistry of angiotensinogen.. Invest. Ophthalmol. Vis. Sci. 1992;33(5):1627-1632.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

The circulating renin-angiotensin system (RAS) is an important determinant in maintaining adequate systemic blood pressure, and it also may modify organ-specific blood flow. All recognized RAS components have been identified in the eye. In this study, angiotensinogen (ANG) was localized using an affinity-purified antibody and paraffin sections of seven human eyes. An antibody for human serum albumin was used for comparison. The ANG was present selectively in the cytoplasm of the nonpigmented ciliary epithelium (NPCE), more prominently in the pars plana than in the pars plicata. Both ANG and albumin were present in the blood vessel lumina of the uvea and retina. Both antibodies also stained perivascular tissue in the uvea, but not in the retina, reflecting the relative tightness of blood-tissue barriers. The detection of ANG in the NPCE may be significant in view of previous descriptions localizing prorenin and angiotensin-converting enzyme in the same cell layer.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×