December 1993
Volume 34, Issue 13
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Articles  |   December 1993
Complete elimination ('cure') of progressively growing intraocular tumors by local injection of tumor-specific CD8+ T lymphocytes.
Author Affiliations
  • S Miki
    Department of Microbiology and Immunology, University of Miami Medical School, Florida 33101.
  • B Ksander
    Department of Microbiology and Immunology, University of Miami Medical School, Florida 33101.
  • J W Streilein
    Department of Microbiology and Immunology, University of Miami Medical School, Florida 33101.
Investigative Ophthalmology & Visual Science December 1993, Vol.34, 3622-3634. doi:
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      S Miki, B Ksander, J W Streilein; Complete elimination ('cure') of progressively growing intraocular tumors by local injection of tumor-specific CD8+ T lymphocytes.. Invest. Ophthalmol. Vis. Sci. 1993;34(13):3622-3634.

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Abstract

PURPOSE: The purpose of this study was to examine if anterior chamber tumors could be eliminated by adoptive immunotherapy. METHODS: Precursor T cells were recovered from lymph nodes draining subconjunctival space or anterior chamber tumors and restimulated in vitro to obtain fully functional tumor-specific CD8+ cytolytic T cells. RESULTS: Subconjunctival space tumor-derived cytolytic T cells eliminated tumors completely when injected simultaneously into the eye with the tumor cells, or when the anterior chamber injection was delayed for up to 3 days. In vitro restimulated cytolytic T cells obtained from lymph nodes draining progressively growing anterior chamber tumors were as effective as those obtained from lymph nodes draining subconjunctival space tumors, indicating that mice with anterior chamber tumors possess precursor cytotoxic T cells with the potential to differentiate into cytolytic T cells capable of eliminating intraocular tumors. By determining the frequency of tumor-specific cytolytic T cells in the effector cell inocula, it was estimated that cures of intraocular tumors are possible when the ratio of cytolytic T cells to tumor cells is less than 1:50, and the total tumor burden at the time of adoptive immunotherapy is less than 80,000 viable tumor cells. CONCLUSIONS: It was concluded that terminally differentiated, antigen-specific, cytotoxic T cells injected directly into an intraocular tumor site are capable of eliminating the tumor, and may represent a mode of ocular tumor immunotherapy in the future.

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