June 1993
Volume 34, Issue 7
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Articles  |   June 1993
Alkali-degraded cornea generates a low molecular weight chemoattractant for polymorphonuclear leukocytes.
Author Affiliations
  • R R Pfister
    Eye Research Laboratories, Brookwood Medical Center, Birmingham, Alabama.
  • J L Haddox
    Eye Research Laboratories, Brookwood Medical Center, Birmingham, Alabama.
  • C I Sommers
    Eye Research Laboratories, Brookwood Medical Center, Birmingham, Alabama.
Investigative Ophthalmology & Visual Science June 1993, Vol.34, 2297-2304. doi:
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      R R Pfister, J L Haddox, C I Sommers; Alkali-degraded cornea generates a low molecular weight chemoattractant for polymorphonuclear leukocytes.. Invest. Ophthalmol. Vis. Sci. 1993;34(7):2297-2304.

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Abstract

PURPOSE: The current study was designed to determine if a polymorphonuclear leukocyte (PMN) chemoattractant is derived from alkali-degraded whole cornea and to establish a range for its molecular weight. METHODS: We utilized a collagen gel-visual chemotactic assay to quantify the directional movement of PMN exposed to alkali-degraded corneas (30 min or 24 hr). In this experiment, the sample to be tested for chemotactic activity passed through a 14,000 molecular weight cutoff membrane into a collagen gel in which resting neutrophils were suspended in a random fashion. Cell movement was videotaped and subsequently tracked by digitizing the centroid of the cells at 30-sec intervals. Computer analysis of these tracks illustrated many behavioral characteristics, including directional movement. RESULTS: Alkali-degraded whole bovine corneas produced a chemotactic response in neutrophils within 5 min. Dilution of the 24-hr sample to 1:7 yielded a significant chemotactic response. The chemotactic response of both the 30-min and 24-hr samples followed a dose-response curve. CONCLUSIONS: This agent may be one of the inflammatory mediators that trigger the early neutrophil response after an alkali-injury to the eye.

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