June 1993
Volume 34, Issue 7
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Articles  |   June 1993
Metallothionein shows an age-related decrease in human macular retinal pigment epithelium.
Author Affiliations
  • D J Tate, Jr
    Sensory and Electrophysiology Research Unit, Touro Infirmary, New Orleans, LA 70115.
  • D A Newsome
    Sensory and Electrophysiology Research Unit, Touro Infirmary, New Orleans, LA 70115.
  • P D Oliver
    Sensory and Electrophysiology Research Unit, Touro Infirmary, New Orleans, LA 70115.
Investigative Ophthalmology & Visual Science June 1993, Vol.34, 2348-2351. doi:
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    • Get Citation

      D J Tate, D A Newsome, P D Oliver; Metallothionein shows an age-related decrease in human macular retinal pigment epithelium.. Invest. Ophthalmol. Vis. Sci. 1993;34(7):2348-2351.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To investigate the possible role of zinc-metallothionein in human retinal pigment epithelium with regard to age-related changes. METHODS: A cadmium/heme assay was used to quantitate metallothionein in isolated macular and peripheral retinal pigment epithelium from donors ranging in age from 28 to 91 yr (n = 16, mean age = 68.6 yr). RESULTS: It was found that peripheral retinal pigment epithelium contained significantly more metallothionein and zinc than macular retinal pigment epithelium. Macular retinal pigment epithelium cells contained 17.6 +/- 2.2 micrograms metallothionein/mg cytosolic protein in donors younger than 70 yr, compared to 5.6 +/- 0.9 in macular retinal pigment epithelium from donors older than 70 yr, a 68% decline (P = 0.0007). In cultured retinal pigment epithelium, when we lowered the zinc concentration in the medium, metallothionein was reduced by 72% after 1 wk of incubation. CONCLUSIONS: It is suggested that lower levels of metallothionein in retinal pigment epithelium are caused by reduced metallothionein gene activity or a faster rate of protein degradation, both of which are known to be regulated, at least partly, by bioavailable zinc.

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