June 1993
Volume 34, Issue 7
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Articles  |   June 1993
The effects of myristyl gamma-picolinium chloride on the rabbit retina: morphologic observations.
Author Affiliations
  • E Zemel
    Department of Physiology and Biophysics, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
  • A Loewenstein
    Department of Physiology and Biophysics, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
  • M Lazar
    Department of Physiology and Biophysics, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
  • I Perlman
    Department of Physiology and Biophysics, Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
Investigative Ophthalmology & Visual Science June 1993, Vol.34, 2360-2366. doi:
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    • Get Citation

      E Zemel, A Loewenstein, M Lazar, I Perlman; The effects of myristyl gamma-picolinium chloride on the rabbit retina: morphologic observations.. Invest. Ophthalmol. Vis. Sci. 1993;34(7):2360-2366.

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Abstract

PURPOSE: This study was designed to localize the site of action of myristyl gamma-picolinium chloride (MGP) in the rabbit retina and to evaluate the extent of the structural damage induced by the drug. METHODS: The structural damage was assessed at the light microscopic level in eyes treated with various concentrations of MGP at different time intervals after intravitreal injection of the drug. Glial fibrillary acidic protein (GFAP) immunoreactivity was tested in the same eyes and served as an index of retinal damage. RESULTS: The rabbit retinas, examined about 1 mo after MGP injection, exhibited loss of photoreceptors and thinning of the retina in the regions close to the site of injection; remote retinal areas appeared morphologically intact or only slightly affected. Immunocytochemical analysis demonstrated the presence of GFAP in Müller (glial) cells throughout the entire retina. When the effects of MGP were examined at short time intervals (24 and 72 hr) after injection, severe morphologic damage in areas adjacent to the site of drug injection developed in parallel with the electroretinographic findings. However, GFAP could not be demonstrated. CONCLUSIONS: MGP, the preservative used in Depo-Medrol (Upjohn, Kalamazoo, MI), is highly toxic to the rabbit retina.

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