August 1993
Volume 34, Issue 9
Free
Articles  |   August 1993
Does topical hydrogen peroxide penetrate the cornea?
Author Affiliations
  • G Wilson
    Vision Science Research Center, School of Optometry, University of Alabama, Birmingham 35294-4390.
  • M V Riley
    Vision Science Research Center, School of Optometry, University of Alabama, Birmingham 35294-4390.
Investigative Ophthalmology & Visual Science August 1993, Vol.34, 2752-2760. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      G Wilson, M V Riley; Does topical hydrogen peroxide penetrate the cornea?. Invest. Ophthalmol. Vis. Sci. 1993;34(9):2752-2760.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: To determine under what conditions hydrogen peroxide (H2O2) can penetrate the cornea and cause increased concentrations of H2O2 in the aqueous humor. METHODS: Rabbit corneas were exposed in vitro and in vivo to H2O2 concentrations up to 60 mmol/l either in a 600 microliter volume or as consecutive drops. H2O2 was measured over time either in the endothelial superfusate or in tapped samples of aqueous humor, and in the fluid applied to the ocular surface. The stability of exogenous H2O2 added directly to aqueous humor was also determined. RESULTS: Exogenous H2O2 in aqueous humor decays with a half-life of 20 minutes, chiefly as a result of catalase activity. When applied to the entire ocular surface, 600 microliters of 60 mmol/l H2O2 caused no change in the concentration in aqueous humor, but when applied to the corneal surface alone, penetration occurred at 18 mmol/l and above. When applied as eight 40-microliters drops to the ocular surface the threshold for H2O2 penetration was above 36 mmol/l. CONCLUSIONS: H2O2 is rapidly eliminated on the ocular surface, chiefly by enzyme activity of the conjunctiva and cornea. Threshold for penetration of H2O2 into the aqueous humor depends on volume, concentration, duration, and ocular surface exposed. In a healthy eye, exogenously derived H2O2 is eliminated by enzyme activity of the aqueous humor and tissues surrounding the anterior chamber.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×