May 1992
Volume 33, Issue 6
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Articles  |   May 1992
EGF, basic FGF, and TGF beta-1 messenger RNA production in rabbit corneal epithelial cells.
Author Affiliations
  • S E Wilson
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
  • S A Lloyd
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
  • Y G He
    Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
Investigative Ophthalmology & Visual Science May 1992, Vol.33, 1987-1995. doi:
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      S E Wilson, S A Lloyd, Y G He; EGF, basic FGF, and TGF beta-1 messenger RNA production in rabbit corneal epithelial cells.. Invest. Ophthalmol. Vis. Sci. 1992;33(6):1987-1995.

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Abstract

The polymerase chain reaction (PCR) was used to demonstrate that rabbit corneal epithelial cells produce messenger RNAs coding for epidermal growth factor (EGF), basic fibroblast growth factor (FGFb), and transforming growth factor beta-1 (TGF beta 1) ex vivo and in primary culture. EGF, FGFb, and TGF beta 1 mRNAs were detected in central and peripheral ex vivo epithelial tissue in wounded and unwounded rabbit corneas. Southern blots of the PCR products were probed with oligonucleotides to demonstrate that the appropriately sized amplification products were specific. These results suggest that corneal epithelial cells produce growth factors that may have autocrine or paracrine effects on epithelial cells, and possibly other cells of the cornea. The functions, if any, performed by these growth factors in corneal epithelial wound healing are yet to be elucidated.

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