August 1996
Volume 37, Issue 9
Free
Articles  |   August 1996
Presence of functional type B natriuretic peptide receptor in human ocular cells.
Author Affiliations
  • I H Pang
    Alcon Laboratories, University of North Texas Health Science Center, Fort Worth, USA.
  • D L Shade
    Alcon Laboratories, University of North Texas Health Science Center, Fort Worth, USA.
  • S Matsumoto
    Alcon Laboratories, University of North Texas Health Science Center, Fort Worth, USA.
  • H T Steely
    Alcon Laboratories, University of North Texas Health Science Center, Fort Worth, USA.
  • L DeSantis
    Alcon Laboratories, University of North Texas Health Science Center, Fort Worth, USA.
Investigative Ophthalmology & Visual Science August 1996, Vol.37, 1724-1731. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      I H Pang, D L Shade, S Matsumoto, H T Steely, L DeSantis; Presence of functional type B natriuretic peptide receptor in human ocular cells.. Invest. Ophthalmol. Vis. Sci. 1996;37(9):1724-1731.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: To study the effects of natriuretic peptides on cyclic guanosine monophosphate (cGMP) production and calcium mobilization in cultured human ocular cells. METHODS: Cultured simian virus 40-transformed (HTM-3) and nontransformed (HTM-16) human trabecular meshwork (TM) cells and nontransformed human ciliary muscle (CM) cells were used. Accumulation of cGMP in cells lysate was measured by radioimmunoassay. Intracellular calcium concentration was measured by microscope-based ratiofluorometry. RESULTS: Both atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) increased the accumulation of cGMP in HTM-3, HTM-16, and CM cells. In the nontransformed TM cells, CNP was five times more efficacious (maximal effect of CNP was 497% +/- 44% that of ANP) and 10 times more potent than ANP (ANP, log [EC50] = -6.99 +/- 0.08; CNP, log [EC50] = -7.96 +/- 0.20). Similar results were seen in HTM-3 and CM cells. Under the assay conditions used, the peptides increased only the production of cGMP without changing its degradation rate. The peptide-induced increase of cGMP in the TM and CM cells correlated with suppression of carbachol-induced calcium mobilization in the cell. CONCLUSIONS: It is known that CNP, but not ANP, selectively activates the guanylyl cyclase associated with the type B natriuretic peptide receptor (NPR-B). Thus, the data suggest that NPR-B is the primary functional NPR in the TM and CM cells. The effects on cGMP and calcium produced by the activation of this receptor are expected to alter TM and CM contractility and may affect aqueous humor hydrodynamics and intraocular pressure.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×