December 1996
Volume 37, Issue 13
Free
Articles  |   December 1996
Topical cyclosporine inhibits mast cell-mediated conjunctivitis.
Author Affiliations
  • S M Whitcup
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
  • C C Chan
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
  • D A Luyo
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
  • P Bo
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
  • Q Li
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.
Investigative Ophthalmology & Visual Science December 1996, Vol.37, 2686-2693. doi:
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    • Get Citation

      S M Whitcup, C C Chan, D A Luyo, P Bo, Q Li; Topical cyclosporine inhibits mast cell-mediated conjunctivitis.. Invest. Ophthalmol. Vis. Sci. 1996;37(13):2686-2693.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: Allergic conjunctivitis is a common condition caused by a mast cell-mediated hypersensitivity reaction to immunoglobulin E-bound allergens. The purpose of this study was to investigate the effect of topical cyclosporine A on the development of mast cell-mediated conjunctivitis in mice. METHODS: Allergic conjunctivitis was induced in C57BL/6 mice by topical applications of compound 48/80, a mast cell degranulating agent. In two separate experiments, mice were treated with topical cyclosporine A (0.05%, 0.2%, or 0.4%), prednisolone acetate 1%, or phosphate-buffered saline. Twenty-four hours after compound 48/80 instillation, the number of neutrophils, eosinophils, lymphocytes, macrophages, and the number of preserved goblet cells and undegranulated mast cells in the conjunctiva were counted by a masked observer. RESULTS: In both experiments, treatment with all three doses of cyclosporine A resulted in a statistically significant reduction in the number of infiltrating neutrophils and eosinophils compared to saline-treated controls. There was no significant difference in the treatment effect of cyclosporine and prednisolone acetate. In addition, there was increased preservation of goblet cells in the cyclosporine A-treated animals. Immunohistochemical staining showed a reduction in infiltrating lymphocytes and a smaller reduction in infiltrating macrophages in animals treated with cyclosporine compared to saline-treated controls. CONCLUSIONS: Topical cyclosporine A was effective in inhibiting the development of mast cell-mediated allergic conjunctivitis in mice. This study suggests that topical cyclosporine A may be effective in treating allergic conjunctivitis in humans.

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