December 1998
Volume 39, Issue 13
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Articles  |   December 1998
Identification of the cornea-specific keratin 12 promoter by in vivo particle-mediated gene transfer.
Author Affiliations
  • A Shiraishi
    Department of Ophthalmology, University of Cincinnati, Ohio 45267-0527, USA.
  • R L Converse
    Department of Ophthalmology, University of Cincinnati, Ohio 45267-0527, USA.
  • C Y Liu
    Department of Ophthalmology, University of Cincinnati, Ohio 45267-0527, USA.
  • F Zhou
    Department of Ophthalmology, University of Cincinnati, Ohio 45267-0527, USA.
  • C W Kao
    Department of Ophthalmology, University of Cincinnati, Ohio 45267-0527, USA.
  • W W Kao
    Department of Ophthalmology, University of Cincinnati, Ohio 45267-0527, USA.
Investigative Ophthalmology & Visual Science December 1998, Vol.39, 2554-2561. doi:
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      A Shiraishi, R L Converse, C Y Liu, F Zhou, C W Kao, W W Kao; Identification of the cornea-specific keratin 12 promoter by in vivo particle-mediated gene transfer.. Invest. Ophthalmol. Vis. Sci. 1998;39(13):2554-2561.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: Keratin 12 (K12) is a cornea epithelial cell-specific intermediate filament component. To provide a better understanding of its expression, it is necessary to identify and characterize the promoter of Krt1.12 gene. METHODS: The 2.5-kb DNA 5' to Krt1.12 gene was sequenced. Krt1.12 promoter-beta-gal DNA constructs were prepared and used in vivo to transfect rabbit corneas, conjunctivas, and skin by particle-mediated gene transfer (Gene Gun). In vitro, the DNA constructs were transfected into cultured T-antigen-transformed rabbit corneal epithelial (RCE-T) cells and human fibrosarcoma HT-1080 fibroblasts with lipofectamine. The promoter activity was assessed by measuring beta-gal (beta-galactosidase) activity using histochemical staining with 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside and enzyme assay with o-nitrophenyl beta-D-galactopyranoside. RESULTS: There are four Pax-6 pair box binding elements found between -910 and -2000 bp 5'-flanking the transcription initiation site of the Krt1.12 gene. None of promoter constricts can be expressed by HT-1080 cells. Cotransfection of Pax-6 cDNA with K12 promoter-beta-gal constructs containing Pax-6 elements results in a fourfold increase of beta-gal activities in RCE-T cells but not HT-1080 fibroblasts. The data of in vivo transfection in the rabbit by Gene Gun indicate that reporter gene constructs containing 0.6-kb and longer DNA fragments 5'-flanking Krt1.12 gene are effectively expressed in corneal, but not conjunctival or epidermal epithelial cells. CONCLUSIONS: The particle-mediated gene transfer is a suitable technique for in vivo delivery of transgenes to corneal epithelial cells. The 2.5-kb DNA fragment 5'-flanking Krt1.12 contains corneal epithelial cell-specific regulatory cis-DNA elements. Pax-6 is a positive transcription factor essential for keratin 12 expression.

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