August 1996
Volume 37, Issue 9
Free
Articles  |   August 1996
Membrane-bound regulators of complement activation in uveal melanomas. CD46, CD55, and CD59 in uveal melanomas.
Author Affiliations
  • W R Goslings
    Department of Ophthalmology, Leiden University Hospital, Netherlands.
  • D J Blom
    Department of Ophthalmology, Leiden University Hospital, Netherlands.
  • I de Waard-Siebinga
    Department of Ophthalmology, Leiden University Hospital, Netherlands.
  • E van Beelen
    Department of Ophthalmology, Leiden University Hospital, Netherlands.
  • F H Claas
    Department of Ophthalmology, Leiden University Hospital, Netherlands.
  • M J Jager
    Department of Ophthalmology, Leiden University Hospital, Netherlands.
  • A Gorter
    Department of Ophthalmology, Leiden University Hospital, Netherlands.
Investigative Ophthalmology & Visual Science August 1996, Vol.37, 1884-1891. doi:
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      W R Goslings, D J Blom, I de Waard-Siebinga, E van Beelen, F H Claas, M J Jager, A Gorter; Membrane-bound regulators of complement activation in uveal melanomas. CD46, CD55, and CD59 in uveal melanomas.. Invest. Ophthalmol. Vis. Sci. 1996;37(9):1884-1891.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To identify the presence of membrane-bound regulators of complement activation (m-RCA) on uveal melanomas and uveal melanoma cell lines and to examine their role in the inhibition of complement-mediated lysis in vitro. METHODS: Immunohistochemistry and flow cytometric analysis with monoclonal antibodies directed against m-RCA CD46, CD55, and CD59 were applied to tissue sections of 10 uveal melanomas, three primary uveal melanoma cell lines, and one uveal melanoma metastatic cell line. A microcytotoxicity test was used for measuring antibody-dependent complement-mediated lysis. RESULTS: The tissue sections and all four uveal melanoma cell lines expressed CD46, CD55, and CD59. Complement-mediated lysis in the presence of human complement was increased after partial removal of the m-RCA CD55 and CD59 with phosphatidylinositol-specific phospholipase C from the uveal melanoma cell line 92-1. CONCLUSIONS: These results demonstrate that CD46, CD55, and CD59 are expressed in uveal melanomas and that CD55 or CD59, or both, plays a role in resistance to complement-mediated cytotoxicity. The finding that m-RCA are expressed in uveal melanomas may have implications for the effectiveness of the anti-tumor response and in the therapeutic application of monoclonal antibodies directed against tumor-associated antigens.

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