August 1996
Volume 37, Issue 9
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Articles  |   August 1996
Localization of TIMP-3 mRNA expression to the retinal pigment epithelium.
Author Affiliations
  • N G Della
    Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287-9289, USA.
  • P A Campochiaro
    Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287-9289, USA.
  • D J Zack
    Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287-9289, USA.
Investigative Ophthalmology & Visual Science August 1996, Vol.37, 1921-1924. doi:
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    • Get Citation

      N G Della, P A Campochiaro, D J Zack; Localization of TIMP-3 mRNA expression to the retinal pigment epithelium.. Invest. Ophthalmol. Vis. Sci. 1996;37(9):1921-1924.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To evaluate ocular sites of expression of the tissue inhibitor of metalloproteinases-3 gene (TIMP-3). METHODS: In situ hybridization was performed on frozen sections of albino mouse eyes using riboprobes generated to the 3' untranslated region of TIMP-3. RESULTS: TIMP-3 mRNA expression was detected strongly in the retinal pigment epithelium (RPE) and to a minor extent in the ciliary epithelium, but not at any other site within the eye. CONCLUSIONS: Expression of TIMP-3 in the RPE is consistent with the recent demonstration of TIMP-3 mutations in patients with Sorsby's fundus dystrophy, a condition marked by the early onset of choroidal neovascularization in the macula. Unlike many of the recently described genes that cause human retinal disease, TIMP-3 is preferentially expressed in the RPE of the normal eye, as opposed to the photoreceptors.

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