July 1996
Volume 37, Issue 8
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Articles  |   July 1996
Confirmation of the identity of the major phospholipid in human lens membranes.
S R Ferguson; D Borchman; M C Yappert
Author Affiliations
  • S R Ferguson
    Department of Chemistry, Kentucky Lions Eye Research Institute, University of Louisville 40292, USA.
  • D Borchman
    Department of Chemistry, Kentucky Lions Eye Research Institute, University of Louisville 40292, USA.
  • M C Yappert
    Department of Chemistry, Kentucky Lions Eye Research Institute, University of Louisville 40292, USA.
Investigative Ophthalmology & Visual Science July 1996, Vol.37, 1703-1706. doi:
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      S R Ferguson, D Borchman, M C Yappert; Confirmation of the identity of the major phospholipid in human lens membranes.. Invest. Ophthalmol. Vis. Sci. 1996;37(8):1703-1706.

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Abstract

PURPOSE: To confirm the identity of the major component of the human lens membranes proposed in 1994 to be dihydrosphingomyelin (DH-SPH). METHODS: DH-SPH was prepared by catalytic hydrogenation of the double bond between carbons 4 and 5 of sphingomyelin (SPH). DH-SPH was characterized by phosphorus-31 (31P) and proton (1H) nuclear magnetic resonance (NMR) spectroscopy at different reaction times. The spectroscopic data were compared to those of the major component extracted from human lens membranes. RESULTS: Both the 1H NMR and the 31P NMR spectral resonances of the prepared DH-SPH matched those for the once "unknown phospholipid" that constitutes approximately half the human lens phospholipids. CONCLUSIONS: The match of the spectroscopic NMR data obtained for the DH-SPH prepared by hydrogenation of SPH and those for the major phospholipid isolated from the human lens membranes confirms the identity of this sphingolipid as D-erythro-4,5-dihydrosphingomyelin.

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