May 1998
Volume 39, Issue 6
Free
Articles  |   May 1998
Age-related lipid oxidation in human lenses.
Author Affiliations
  • D Borchman
    Department of Ophthalmology and Visual Sciences, University of Louisville, Kentucky 40202, USA.
  • M C Yappert
    Department of Ophthalmology and Visual Sciences, University of Louisville, Kentucky 40202, USA.
Investigative Ophthalmology & Visual Science May 1998, Vol.39, 1053-1058. doi:
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      D Borchman, M C Yappert; Age-related lipid oxidation in human lenses.. Invest. Ophthalmol. Vis. Sci. 1998;39(6):1053-1058.

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Abstract

PURPOSE: To quantify age-related changes in products of lipid oxidation in human lenses and to relate these changes to membrane hydrocarbon chain structure. Deviation from a well-defined membrane-lipid composition and structure could result in alterations in membrane function and disruption of the homeostasis of the cell. METHODS: Infrared spectroscopy was used to detect lipid compositional and structural changes in human lens membranes associated with age and cataracts. RESULTS: Lipid oxidation increased linearly threefold relative to total phospholipids in subjects ranging in age between 1 and 85 years, as was evident by increases in trans double bonds, lipid carbonyls, and secondary products. There was no statistical difference between the levels of lipid oxidation in the cortex or nucleus. Lipid hydrocarbon chain order (rigidity) increased from approximately 40% at birth to 70% at 80 years of age. Changes in lipid order correlated with changes in the relative content of membrane phosphatidylcholine and sphingomyelin, and with the level of lipid oxidation. CONCLUSIONS: Lipid oxidation increased linearly and uniformly throughout the human lens with age. The change in lipid oxidation with age correlated to a change in lipid order.

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