January 1999
Volume 40, Issue 1
Free
Articles  |   January 1999
Regulation of human leukocyte antigen expression in human conjunctival epithelium.
Author Affiliations
  • K Tsubota
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
  • K Fukagawa
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
  • T Fujihara
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
  • S Shimmura
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
  • I Saito
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
  • K Saito
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
  • T Takeuchi
    Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
Investigative Ophthalmology & Visual Science January 1999, Vol.40, 28-34. doi:
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      K Tsubota, K Fukagawa, T Fujihara, S Shimmura, I Saito, K Saito, T Takeuchi; Regulation of human leukocyte antigen expression in human conjunctival epithelium.. Invest. Ophthalmol. Vis. Sci. 1999;40(1):28-34.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To demonstrate that interferon-gamma (IFN-gamma) is the key cytokine responsible for the upregulation of HLA-DR antigen in conjunctival epithelial cells of Sjogren syndrome (SS) patients. METHODS: Flow cytometry of conjunctival epithelial cells from SS and non-SS dry eye patients was performed for the quantification of HLA-DR surface expression. With a conjunctival epithelial cell line (ChWK), HLA-DR regulation by various cytokines was evaluated, and confocal immunocytochemical and western blot analyses were performed to evaluate the activation of nuclear factorkappa B (NF-kappaB) and signal transducers and activators of transcription 1 and 3 (STAT1 and STAT3, respectively). RESULTS: HLA-DR expression was upregulated in conjunctival epithelial cells of SS patients but not in non-SS dry eye patient or healthy control subject. IFN-gamma was the only cytokine that effectively upregulated HLA-DR expression in ChWK, which was synergistically enhanced by tumor necrosis factor-alpha (TNF-alpha). IFN-gamma induced the nuclear translocation of NF-kappaB, but did not activate STAT1 or STAT3 in ChWK. CONCLUSIONS: Upregulation of HLA-DR antigen in the conjunctival epithelium of SS patients may be regulated by IFN-gamma through the activation of NF-kappaB.

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