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Satoru Yamagami, Tadahiko Tsuru; Increase in Orthotopic Murine Corneal Transplantation Rejection Rate with Anterior Synechiae. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2422-2426.
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purpose. To evaluate the immunologic effect of anterior synechiae (AS) in a murine
model of corneal transplantation.
methods. Orthotopic penetrating keratoplasty with 12 interrupted sutures was
performed on C57BL/6 donor mice and BALB/c recipient mice without AS
(AS− group). In contrast to suturing in the AS− group, 3 of the 12
sutures were placed to create AS (AS+ group). The average graft opacity
scores and rejection rates of both groups were compared. Cytotoxic
T-lymphocyte (CTL) reactions and delayed hypersensitivity (DH) were
evaluated 3 weeks after transplantation. Corneal cytokine expression
results. The opacity scores of the AS+ group were consistently greater than
those of the AS− group, and the rejection rate of the AS+ group was
significantly greater than that of the AS− group (86% versus 54%, P = 0.03). The AS+ group had significantly higher
CTL activity compared with the AS− group. There was no significant
difference in DH between the two groups. The cytokine expression
pattern in the AS+ group became similar to that of the AS− group in
which the grafts were rejected.
conclusions. These findings indicate that AS impairs ocular immune privilege by
mediating CTL activity, but without intensifying the DH response.
Therefore, AS is a critical risk factor in allograft rejection in a
murine model of corneal transplantation.
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