Purchase this article with an account.
Chie Sotozono, Jiucheng He, Mamoru Tei, Yoich Honma, Shigeru Kinoshita; Effect of Metalloproteinase Inhibitor on Corneal Cytokine Expression after Alkali Injury. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2430-2434.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. Interleukin (IL)-1α and IL-6 levels in the cornea are greatly elevated during the
early stages after an alkali burn in mice. The authors investigated the
effect of synthetic inhibitor of matrix metalloproteinase (SIMP) on the
expression of inflammatory cytokines in alkali-burned murine corneas
and evaluated the clinical appearance of the eyes.
methods. After 0.5N NaOH-alkali burns to 400 corneas of ICR mice, 200 received
400 μg/ml of SIMP topically 4 times a day while 200 corneas were
similarly treated with vehicle only. At days 4, 7 and 14 after injury,
each cornea was assigned a clinical score for corneal opacity, corneal
epithelial defect, hyphema and cataract. Extracts of injured corneas in
each group were then assayed for cytokine production using ELISA
systems for IL-1α, IL-1β, IL-6 and tumor necrosis factor-α
results. The levels of IL-1α, IL-1β and IL-6 were significantly lower in the
SIMP-treated group than in the vehicle-treated group 7 days after the
burn. However, levels of these cytokines were similar in the SIMP and
non-SIMP groups at days 4 and 14. Levels of TNF-α did not differ
between both groups at any postinjury time. In the SIMP-treated
corneas, there was less opacification and hyphema formation and
epithelial regeneration was faster.
conclusions. Topical application of SIMP in alkali-burned murine corneas reduced the
expression of IL-1α, IL-1β, and IL-6 and lessened the severity of
This PDF is available to Subscribers Only