April 1999
Volume 40, Issue 5
Free
Articles  |   April 1999
Latrunculin-A increases outflow facility in the monkey.
Author Affiliations
  • J A Peterson
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
  • B Tian
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
  • A D Bershadsky
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
  • T Volberg
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
  • R E Gangnon
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
  • I Spector
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
  • B Geiger
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
  • P L Kaufman
    Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, USA.
Investigative Ophthalmology & Visual Science April 1999, Vol.40, 931-941. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      J A Peterson, B Tian, A D Bershadsky, T Volberg, R E Gangnon, I Spector, B Geiger, P L Kaufman; Latrunculin-A increases outflow facility in the monkey.. Invest. Ophthalmol. Vis. Sci. 1999;40(5):931-941.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: To determine the effect of Latrunculin (LAT)-A, a macrolide that binds to G-actin, which leads to the disassembly of actin filaments, on shape, junctions, and the cytoskeleton of cultured bovine aortic endothelial cells (BAECs) and on outflow facility in living monkeys. METHODS: Latrunculin-A dose-time-response relationships in BAECs were determined by immunofluorescence and phase contrast light microscopy, facility by two-level constant pressure anterior chamber perfusion. RESULTS: In BAECs, LAT-A caused dose- and incubation time- dependent destruction of actin bundles, cell separation, and cell loss. Cell-cell adhesions were more sensitive than focal contacts. Recovery was also dose- and time-dependent. In monkeys, exchange intracameral infusion and topical application of LAT-A induced dose- and time-dependent several-fold facility increases. The facility increase was completely reversed within several hours after drug removal. However, for at least 24 hours after a single topical LAT-A dose, perfusion with drug-free solution caused an accelerated increase in facility beyond that attributed to normal resistance washout. CONCLUSIONS: Pharmacological disorganization of the actin cytoskeleton in the trabecular meshwork by specific actin inhibitors like LAT-A may be a useful antiglaucoma strategy.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×