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Deeba Husain, Michal Kramer, Alice G. Kenny, Norman Michaud, Thomas J. Flotte, Evangelos S. Gragoudas, Joan W. Miller; Effects of Photodynamic Therapy Using Verteporfin on Experimental Choroidal Neovascularization and Normal Retina and Choroid up to 7 Weeks after Treatment. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2322-2331.
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purpose. To study the long-term effects of photodynamic therapy (PDT), using
liposomal benzoporphyrin derivative (BPD) or Verteporfin, on
experimental choroidal neovascularization (CNV) and on normal retina
and choroid (with no CNV) in the cynomolgus monkey eye.
methods. Photodynamic therapy was performed in 8 cynomolgus monkey eyes with
experimental CNV induced by laser injury. The effect of PDT on normal
retina and choroid (with no CNV) was studied in 9 monkey eyes.
Liposomal BPD was administered intravenously (0.375 mg/kg) either as a
bolus, as a slow infusion over 32 minutes, or as a fast infusion over
10 minutes. Photodynamic therapy was performed using light at a
wavelength of 689 or 692 nm, with an irradiance of 600
mW/cm2 and fluence of 150 J/cm2. Follow-up
studies, including fundus photography and FA, were performed at 24
hours after PDT and then weekly. Indocyanine green and BPD angiography
were performed in selected cases. Tissues were examined with light and
electron microscopy at the end of follow-up.
results. Twenty-three of the 32 areas of CNV treated with PDT showed absence of
angiographic leakage at 24 hours. Twenty-eight areas of CNV were
followed for 4 weeks; 22 of 28 showed absence of angiographic leakage
at 2 weeks; and 20 of 28 at 4 weeks of follow-up. Forty spots on the
normal retina and choroid were treated with PDT and were followed for 4
to 7 weeks. These spots showed pigment-laden cells in the outer retina,
variably pigmented retinal pigment epithelium (RPE) in the treated
area, intact neurosensory retina, and reperfusion of the
conclusions. Photodynamic therapy leads to absence of angiographic leakage for at
least 4 weeks in experimental CNV in the monkey model. In the normal
monkey eye the RPE and choriocapillaris show generalized recovery with
preservation of the neurosensory retina 7 weeks after
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