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Lone Helboe, Morten Møller; Immunohistochemical Localization of Somatostatin Receptor Subtypes sst1 and sst2 in the Rat Retina. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2376-2382.
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purpose. To investigate the distribution of somatostatin receptor subtypes
sst1 and sst2 in the rat retina by
immunohistochemistry and to characterize further the neurotransmitters
of the sst1- and sst2-immunoreactive cells.
methods. Polyclonal antibodies raised against sst1 and
sst2 receptors were applied to 12-μm cryostat sections of
rat retinas fixed in paraformaldehyde. Further, immunofluorescence
double labeling was performed for the sst1 and
sst2 receptors with somatostatin, tyrosine hydroxylase (TH)
and glutamate decarboxylase (GAD).
results. Immunoreactivity for sst1 was present in somatostatinergic
amacrine cells located in the inner nuclear layer (INL) and in
displaced amacrine cells in the ganglion cell layer of the retina.
Also, a small number of ganglion cells were sst1 immunoreactive. Immunoreactivity for sst2 was observed in
many medium-sized amacrine cells in the middle part of the INL, with a
central process projecting to the sublaminae of the inner plexiform
layer. Furthermore, sst2 immunoreactivity was found in
large amacrine cells of the INL. These cells also contained TH. Inner
segments of cone receptors were stained with the sst2 antiserum. Immunostaining for sst2, and to a minor extent
for sst1, was found in Müller cell fibers. None of
the somatostatin receptors colocalized with GAD.
conclusions. These findings suggest that the sst1 receptor may function
as an autoreceptor on retinal somatostatinergic cells. The presence of
sst2 receptors on the TH-immunoreactive amacrine cells
indicates an influence of somatostatin on the secretion of dopamine in
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