April 1999
Volume 40, Issue 5
Free
Articles  |   April 1999
Retinal TUNEL-positive cells and high glutamate levels in vitreous humor of mutant quail with a glaucoma-like disorder.
Author Affiliations
  • O Dkhissi
    Laboratoire de NeuroCytologie Oculaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
  • E Chanut
    Laboratoire de NeuroCytologie Oculaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
  • M Wasowicz
    Laboratoire de NeuroCytologie Oculaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
  • M Savoldelli
    Laboratoire de NeuroCytologie Oculaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
  • J Nguyen-Legros
    Laboratoire de NeuroCytologie Oculaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
  • F Minvielle
    Laboratoire de NeuroCytologie Oculaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
  • C Versaux-Botteri
    Laboratoire de NeuroCytologie Oculaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
Investigative Ophthalmology & Visual Science April 1999, Vol.40, 990-995. doi:
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    • Get Citation

      O Dkhissi, E Chanut, M Wasowicz, M Savoldelli, J Nguyen-Legros, F Minvielle, C Versaux-Botteri; Retinal TUNEL-positive cells and high glutamate levels in vitreous humor of mutant quail with a glaucoma-like disorder.. Invest. Ophthalmol. Vis. Sci. 1999;40(5):990-995.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To investigate whether retinal cell death observed in an avian glaucoma-like disorder occurs by apoptosis and whether an increase in excitotoxic amino acid concentration in the vitreous humor is associated temporally with cell death in the retina. METHODS: Presumptive retinal apoptotic nuclei were identified by histochemical detection of DNA fragmentation (by TdT-dUTP terminal nick-end labeling [TUNEL]), and vitreal concentrations of glutamate and several other amino acids were determined by high-pressure liquid chromatography with fluorometric detection in the al mutant quail (Coturnix coturnix japonica) in which a glaucoma-like disorder develops spontaneously. RESULTS: TUNEL-labeled nuclei were located mostly in the ganglion cell layer (GCL) in the retina of mutant quails 3 months after hatching. However, labeled nuclei were also observed in the inner and outer nuclear layers. At 7 months, most TUNEL-positive nuclei were detected in the inner nuclear layer, whereas labeled cells in the GCL were reduced in number. No TUNEL-labeled nuclei were detected in the retina of control quails at any age. Vitreal concentrations of glutamate and aspartate were significantly increased in 1-month-old mutant quails compared with control animals. Concentrations decreased at 3 months, and no significant differences were observed between strains at 7 months. CONCLUSIONS: Presumptive apoptotic cell death is detected from 3 months after hatching in mutant quails and is not restricted to retinal ganglion cells. Cell death appears just after a significant increase in excitotoxic amino acid concentrations in the vitreous humor, suggesting a correlation between both events.

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