May 1999
Volume 40, Issue 6
Free
Articles  |   May 1999
Retinal ischemia-reperfusion injury attenuated by blocking of adhesion molecules of vascular endothelium.
Author Affiliations
  • A Tsujikawa
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
  • Y Ogura
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
  • N Hiroshiba
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
  • K Miyamoto
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
  • J Kiryu
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
  • S J Tojo
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
  • M Miyasaka
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
  • Y Honda
    Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Japan.
Investigative Ophthalmology & Visual Science May 1999, Vol.40, 1183-1190. doi:
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    • Get Citation

      A Tsujikawa, Y Ogura, N Hiroshiba, K Miyamoto, J Kiryu, S J Tojo, M Miyasaka, Y Honda; Retinal ischemia-reperfusion injury attenuated by blocking of adhesion molecules of vascular endothelium.. Invest. Ophthalmol. Vis. Sci. 1999;40(6):1183-1190.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To evaluate quantitatively the effects of blocking of adhesion molecules (P-selectin or intercellular adhesion molecule-1 [ICAM-1]) on leukocyte dynamics in the retinal microcirculation in vivo during ischemia-reperfusion injury and the therapeutic efficacy of the blocking of adhesion molecules on retinal ischemia-reperfusion injury. METHODS: Retinal ischemia was induced for 60 minutes in anesthetized pigmented rats by temporary ligation of the optic nerve. P-selectin or ICAM-1 monoclonal antibody (mAb) was administered at 5 minutes before reperfusion. At 4, 12, and 24 hours after onset of reperfusion, leukocyte behavior in the retinal microcirculation was evaluated in vivo with acridine orange digital fluorography. After 7 or 14 days of reperfusion, retinal damage was evaluated histologically. RESULTS: P-selectin mAb significantly inhibited leukocyte rolling along the major retinal veins after reperfusion. Subsequently, the number of accumulated leukocytes decreased in the P-selectin-inhibited rats. ICAM-1 mAb also inhibited leukocyte accumulation during the reperfusion period in a more substantial manner than P-selectin mAb. Histologic examination demonstrated the protective effect of the blocking of P-selectin or ICAM-1. In accordance with a reduction in leukocyte accumulation, the protective effect of mAb on retinal ischemia-reperfusion injury was more substantial in ICAM-1-inhibited rats. CONCLUSIONS: The present study demonstrates the inhibitory effect of P-selectin and ICAM-1 mAb on leukocyte accumulation and subsequent tissue injury during retinal ischemia-reperfusion injury.

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