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Masatsugu Nakamura, Takashi Nagano, Tai-ichiro Chikama, Teruo Nishida; Role of the Small GTP-Binding Protein Rho in Epithelial Cell Migration in the Rabbit Cornea. Invest. Ophthalmol. Vis. Sci. 2001;42(5):941-947.
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purpose. To determine the role of the small guanosine triphosphate (GTP)-binding
protein Rho in the migration of corneal epithelial cells.
methods. The presence of the Rho target proteins Rho-associated coiled
coil-containing protein kinase (ROCK)-1 and ROCK-2 in rabbit cornea was
examined by immunohistochemical analysis, and that of the corresponding
mRNAs in rabbit corneal epithelial cells was determined by reverse
transcription–polymerase chain reaction analysis. The effects of
various agents on epithelial cell migration were investigated by
measuring the length of the migration path in rabbit corneal blocks in
results. Both ROCK-1 and ROCK-2 were detected in the rabbit corneal epithelium
at both protein and mRNA levels. The Rho activator lysophosphatidic
acid (LPA) stimulated corneal epithelial migration in a dose-dependent
manner, whereas exoenzyme C3, a Rho inhibitor, inhibited epithelial
migration also in a dose-dependent manner. The stimulatory effect of
LPA on corneal epithelial migration was prevented by exoenzyme C3. Both
cytochalasin B, an inhibitor of actin filament assembly, and ML-7, an
inhibitor of myosin light chain kinase, also prevented LPA stimulation
of epithelial migration.
conclusions. These results suggest that Rho mediates corneal epithelial migration in
response to external stimuli by regulating the organization of the
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