March 1999
Volume 40, Issue 3
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Articles  |   March 1999
Increase in the advanced glycation end product pentosidine in Bruch's membrane with age.
Author Affiliations
  • J T Handa
    Department of Ophthalmology, University of California, Davis 95616-8794, USA.
  • N Verzijl
    Department of Ophthalmology, University of California, Davis 95616-8794, USA.
  • H Matsunaga
    Department of Ophthalmology, University of California, Davis 95616-8794, USA.
  • A Aotaki-Keen
    Department of Ophthalmology, University of California, Davis 95616-8794, USA.
  • G A Lutty
    Department of Ophthalmology, University of California, Davis 95616-8794, USA.
  • J M te Koppele
    Department of Ophthalmology, University of California, Davis 95616-8794, USA.
  • T Miyata
    Department of Ophthalmology, University of California, Davis 95616-8794, USA.
  • L M Hjelmeland
    Department of Ophthalmology, University of California, Davis 95616-8794, USA.
Investigative Ophthalmology & Visual Science March 1999, Vol.40, 775-779. doi:
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    • Get Citation

      J T Handa, N Verzijl, H Matsunaga, A Aotaki-Keen, G A Lutty, J M te Koppele, T Miyata, L M Hjelmeland; Increase in the advanced glycation end product pentosidine in Bruch's membrane with age.. Invest. Ophthalmol. Vis. Sci. 1999;40(3):775-779.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

PURPOSE: To determine whether there is an age-related increase of pentosidine in human Bruch's membranes and to localize pentosidine and carboxymethyllysine (CML), two well-characterized, advanced glycation end products (AGEs) in aged human Bruch's membranes and choroid in vivo. METHODS: Human Bruch's membrane samples were isolated from the retinal pigment epithelium (RPE) and choroid and subjected to reversed-phase high-performance liquid chromatography to determine pentosidine content. A polyclonal anti-pentosidine antibody and a monoclonal antibody specific for carboxymethyllysine were used to localize AGEs in 20-month-old nondiabetic, 82-year-old nondiabetic, and 82-year-old diabetic globes. RESULTS: Human Bruch's membranes (n = 20) showed a linear age-dependent increase in pentosidine that reached approximately 0.17 millimoles pentosidine per mole hydroxyproline in late life (r = 0.896; P < 0.001). Immunohistochemical evaluation showed evidence of pentosidine in Bruch's membrane, choroidal extracellular matrix, and vessel walls in the 82-year-old nondiabetic and diabetic globes. A similar staining pattern was found with the anti-CML antibody. Basal laminar deposits and drusen stained with both antibodies in the elderly nondiabetic eye. In contrast, neither antibody stained the 20-month-old tissue. CONCLUSIONS: We provide biochemical and immunohistochemical evidence for the formation of pentosidine and CML structures in human Bruch's membrane and choroid with age. These changes could promote aging of the RPE-Bruch's membrane-choroid complex.

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