Purchase this article with an account.
Steven E. Wilson, Qianwa Liang, Woo Jung Kim; Lacrimal Gland HGF, KGF, and EGF mRNA Levels Increase after Corneal Epithelial Wounding. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2185-2190.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To evaluate the effect of corneal epithelial wounding on lacrimal gland
expression of hepatocyte growth factor (HGF), keratinocyte growth
factor (KGF), and epidermal growth factor (EGF) in the rabbit model.
methods. Rabbits had corneal epithelial scrape injuries, and the lacrimal gland
was removed at different times after wounding. HGF, KGF, and EGF mRNA
expression was examined by quantitative RNase protection assay. HGF,
KGF, and EGF proteins were detected in rabbit lacrimal tissue using
immunoprecipitation and western blot analysis.
results. HGF mRNA and EGF mRNA were significantly increased in rabbit lacrimal
gland tissue within 8 hours after corneal epithelial injury. The
increase in KGF mRNA expression was small and reached significance 1
day after corneal injury. Lacrimal gland expression peaked at 3 days
after wounding for each growth factor mRNA, the same day, on average,
that the epithelial defect healed. After the peak increase in
expression, there was a progressive decline in expression of each
growth factor mRNA, but production was still increased compared with
prewound levels. HGF protein, KGF protein, and EGF proteins were
detected in rabbit lacrimal gland tissue.
conclusions. Levels of HGF, KGF, and EGF mRNAs increase in rabbit lacrimal gland
tissue in response to corneal epithelial wounding. The results of this
study are consistent with the existence of a cornea–nervous
system–lacrimal gland regulatory loop modulating expression of these
growth factor mRNAs. The lacrimal gland is a likely source of increased
HGF and EGF proteins detected in tears in previous
This PDF is available to Subscribers Only