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Masaru Inatani, Hidenobu Tanihara, Atsuhiko Oohira, Megumi Honjo, Yoshihito Honda; Identification of a Nervous Tissue–Specific Chondroitin Sulfate Proteoglycan, Neurocan, in Developing Rat Retina. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2350-2359.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. To identify the expression of neurocan, a nervous tissue–specific
chondroitin sulfate proteoglycan, in retina and to elucidate its
changes during development.
methods. Expressional changes of neurocan mRNAs in developing rat retinas were
investigated by a semiquantitative reverse transcription–polymerase
chain reaction (RT–PCR). The localization and characterization of
neurocan core proteins were also investigated with the use of Western
blot analysis and immunohistochemistry.
results. Gene expression of neurocan was identified in retinas by RT–PCR.
Semiquantitative analysis using Southern blot analysis revealed that
mRNA expression for neurocan increased at increasing postnatal stages
and that it reached its peak around postnatal day 7 (P7).
Immunohistochemical studies demonstrated that in differentiating rat
retinal (neuroblast) cells weak neurocan immunoreactivities were
observed throughout the retina on embryonal days 14 (E14) and E16.
During the early postnatal period, the immunoreactivities became most
conspicuous in the inner and outer plexiform layers on P7 through P14.
In adult retinas, only faint immunostaining was detected. Immunoblot
analysis showed two positive bands of 220- and 150-kDa core
glycoproteins after treatment with chondroitinase ABC. Further
immunoblot analysis revealed that the expression of these two
immunolabeled variants was regulated differently during retinal
conclusions. The temporal and spatial regulation of expression of neurocan and its
proteolytic variant during retinal development suggest that it may play
a role in differentiation and neural network
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