March 1999
Volume 40, Issue 3
Free
Articles  |   March 1999
Human diabetic neovascular membranes contain high levels of urokinase and metalloproteinase enzymes.
Author Affiliations
  • A Das
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
  • P G McGuire
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
  • C Eriqat
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
  • R R Ober
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
  • E DeJuan, Jr
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
  • G A Williams
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
  • A McLamore
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
  • J Biswas
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
  • D W Johnson
    Department of Surgery, University of New Mexico, Veterans Administration Medical Center, Albuquerque 87131, USA.
Investigative Ophthalmology & Visual Science March 1999, Vol.40, 809-813. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      A Das, P G McGuire, C Eriqat, R R Ober, E DeJuan, G A Williams, A McLamore, J Biswas, D W Johnson; Human diabetic neovascular membranes contain high levels of urokinase and metalloproteinase enzymes.. Invest. Ophthalmol. Vis. Sci. 1999;40(3):809-813.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

PURPOSE: Retinal neovascularization is one of the leading causes of blindness. A crucial event in this process is the remodeling and penetration of the capillary basement membrane by migrating endothelial cells. This process requires proteolysis of basement membrane components by a variety of proteinases. The objective of the present study was to determine the expression of proteinases in human retinal tissues showing active neovascularization. METHODS: Epiretinal neovascular membranes surgically removed from patients with proliferative diabetic retinopathy were analyzed by zymography, and the types and amounts of proteinases present in the tissues were determined. Retinas from nondiabetic donor eyes served as control specimens. RESULTS: Both the high- (54 kDa) and low- (33 kDa) molecular-weight forms of urokinase were present at significantly higher levels in neovascular membranes than in normal retinas. The pro forms of the matrix metalloproteinases (MMP) MMP-2 and MMP-9 were significantly elevated in the neovascular membranes in comparison with levels in normal retinas. In addition, the active forms of these enzymes were present in the membranes, whereas there was no detectable level of the active forms in normal retinas. CONCLUSIONS: Human diabetic neovascular membranes contain high levels of urokinase and MMP. The increased activity of proteinases in the final common pathway of retinal neovascularization indicates that inhibition of these enzymes may be a useful therapeutic target as an alternative approach in the management of proliferative retinopathies.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×