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Fabián Benencia, María C. Courrèges, Gisela Gamba, Hernán Cavalieri, Ernesto J. Massouh; Effect of Aminoguanidine, a Nitric Oxide Synthase Inhibitor, on Ocular Infection with Herpes Simplex Virus in Balb/c Mice. Invest. Ophthalmol. Vis. Sci. 2001;42(6):1277-1284.
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purpose. To study the effect of aminoguanidine (AMG), an inhibitor of nitric
oxide production, on the ocular infection of Balb/c mice with herpes
simplex virus (HSV) type 1 strain F and HSV-2 strain G.
methods. Animals were treated with different amounts of AMG (0.5, 0.1, and 0.05
mg/mouse) by topical application in the eye from postinfection (PI)
days −2 through +5, considering 0 the day of infection. At different
PI days, development of herpetic keratitis was evaluated in treated and
results. Treated animals showed a dose-dependent increase in ocular disease
after viral infection, compared with control animals. Viral titers in
ocular washings were higher in AMG-treated mice (PI day 2, HSV-1: AMG
0.5 mg, 1.3 × 103 plaque-forming units (PFU)/ml;
control, 0. 22 × 102 PFU/ml, P <
0.025). At PI day 3, control corneas had only scattered inflammatory
cells, whereas those from treated animals showed a conspicuous
infiltrate consisting primarily of neutrophils. Viral titers were also
higher in brains of treated mice. These animals died earlier and in a
greater proportion than control animals (percentage of mortality, PI
day 12, HSV-1: AMG 0.5 mg, 40% ± 4%; control, 18% ± 3%, P < 0.05).
conclusions. These data indicate an inhibitory effect of nitric oxide on HSV ocular
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