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Mei-Zi Quan, Jun Kosaka, Masami Watanabe, Taketoshi Wakabayashi, Yutaka Fukuda; Survival of Axotomized Retinal Ganglion Cells in Peripheral Nerve–Grafted Ferrets. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2360-2366.
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purpose. Peripheral nerve (PN) grafting to the optic nerve stump stimulates not
only axonal regeneration of the axotomized retinal ganglion cells
(RGCs) into the grafted PN but also their survival. The purpose of the
present study was to determine the number, distribution, and soma
diameter of only surviving RGCs without regenerated axons and surviving
RGCs with regenerated axons in PN-grafted mammals.
methods. A segment of PN was grafted to the optic nerve stump of adult ferrets.
Two months after the PN grafting, surviving RGCs with regenerated axons
were retrogradely labeled with granular blue (GB) and stained with
RGC-specific antibody C38. Surviving RGCs without regenerated axons
were identified as C38-positive cells without GB labeling.
results. Twenty-one percent of RGCs survived axotomy after PN grafting in the
area centralis (AC), whereas 47% survived in the peripheral retina.
Twenty-six percent of surviving RGCs in the AC exhibited axonal
regeneration, which was higher than that in the peripheral retina. Soma
diameter histograms revealed that RGCs with regenerated axons showing
both GB and C38 positivity were in the large soma diameter ranges. In
contrast, the soma diameter distribution of surviving RGCs that did not
have regenerated axons showed a peak in the smaller soma diameter
conclusions. The present data suggest that PN grafting promotes survival of
axotomized RGCs more effectively in the peripheral retina than in the
AC. Among surviving RGCs, the larger cells exhibited axonal
regeneration into the grafted PN, whereas the axons of smaller cells
did not to regenerate in either the AC or the peripheral
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