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Andreas U. Bayer, Thomas Neuhardt, Albrecht C. May, Peter Martus, Klaus-Peter Maag, Scott Brodie, Elke Lütjen–Drecoll, Steven M. Podos, Thom Mittag; Retinal Morphology and ERG Response in the DBA/2NNia Mouse Model of Angle-Closure Glaucoma. Invest. Ophthalmol. Vis. Sci. 2001;42(6):1258-1265. doi: https://doi.org/.
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purpose. To document the time course of retinal dysfunction by scotopic
electroretinography (ERG) and by quantitative morphology in eyes of the
DBA/2NNia substrain of mouse (DBA) with inherited angle-closure
methods. DBA and control C57BL/6J (C57) mice were studied by ERG recordings from
5 to 15 months of age, and by morphology from 1 to 14 months of age.
Scotopic ERGs were simultaneously recorded from both eyes of
dark-adapted anesthetized mice. Changes in the central neuronal retina
were evaluated by quantitative morphometry performed on serial semithin
sections of Epon-embedded eyes.
results. When compared with normal C57 mice, DBA mice showed significant
reductions of the a-wave and b-wave amplitudes by 7 to 8 months, and
the decline continued as the animals aged. The b-wave implicit time in
DBA mice showed a gradual prolongation beginning at 8 months of age,
when compared with C57 mice. Logistic regression analyses revealed
significant correlations in a- and b-wave amplitude reductions between
ipsilateral and contralateral eyes of DBA mice at ages when ERG
parameters were greatly altered. Morphologically, thinning of the whole
retina was already evident in DBA mice at 4 months of age, but loss of
ganglion cells and thinning of the outer plexiform layer were first
seen in 7- to 8-month-old animals. These changes progressed to the end
of the 13-month period studied.
conclusions. Progressive thinning of the outer retinal layers in DBA mice was found
to correlate with decreases in ERG a- and b-wave amplitudes, both
occurring from the age of 7 to 8 months onward. Similarities with the
findings in human late-stage glaucomatous retinopathy indicate the
relevance of this animal model in further glaucoma
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