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Hideho Ueda, Takeshi Baba, Kenji Kashiwagi, Hiroyuki Iijima, Shinichi Ohno; Dystrobrevin Localization in Photoreceptor Axon Terminals and at Blood–Ocular Barrier Sites. Invest. Ophthalmol. Vis. Sci. 2000;41(12):3908-3914.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. Dystrobrevin is a newly discovered dystrophin-associated protein with
multiple sites for phosphorylation on tyrosine residues. In the present
study, the cellular distribution and subcellular localization of
dystrobrevin were examined in the adult rat retina, cornea, lens, iris,
ciliary body, and cultured Müller cells.
methods. Immunoblot analysis, confocal laser scanning microscopy, and
immunoelectron microscopy were used to examine dystrobrevin expression.
results. Immunoblot analysis showed that an approximately 87-kDa band was
expressed predominantly in the lens, retina, iris and ciliary body,
whereas an approximately 60-kDa band was expressed in cultured
Müller cells, cornea, retina, iris, and ciliary body. Confocal
microscopy demonstrated dystrobrevin in the inner limiting membrane,
outer plexiform layer, and retinal pigment epithelium and around blood
vessels in the retina. At the ultrastructural level, dystrobrevin was
localized under cell membranes of rod spherules and cone pedicles of
photoreceptor cell terminals but often was found in the cytoplasm of
endothelial cells and Müller cells. Furthermore, dystrobrevin was
colocalized with β-dystroglycan in corneal endothelium; lens, iris,
and ciliary epithelia; and cultured Müller cells.
conclusions. The present study demonstrates that dystrobrevin is expressed in
neurons, glia, and endothelial cells in the rat retina. In addition,
dystrobrevin is localized at the blood–ocular barrier sites in
extraocular tissue. These data suggest that dystrobrevin plays an
important role in visual function.
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