Purchase this article with an account.
Devinder Singh Chauhan, John Marshall; The Interpretation of Optical Coherence Tomography Images of the Retina. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2332-2342.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To determine the relationship between optical coherence tomography
(OCT) images of the retina and retinal substructure in vitro and in
methods. In vitro, OCT images of human and bovine retina were acquired after
sequential excimer laser ablation of the inner retinal layers.
Measurements of bands in the OCT images were compared with measurements
of retinal layers on histology of the ablated specimens. In vivo, OCT
images were acquired of retinal lesions in which there was a
displacement of pigmented retinal pigment epithelial (RPE) cells:
retinitis pigmentosa and laser photocoagulation (eight eyes each).
results. The mean thickness of human inner OCT bands (131 μm; 95% confidence
interval [CI], 122–140 μm) was 7.3 times that of the retinal nerve
fiber layer (RNFL). This band persisted despite ablation greater than
140 μm. The inner aspect of the outer OCT band corresponded to the
apical RPE, but the mean thickness of this band in human tissue (55μ
m; 95% CI, 48–62 μm) was 2.6 times the thickness of the
RPE–choriocapillaris complex. OCT measurement of total retinal
thickness was accurate (coefficient of variance, 0.05) and precise
(coefficient of correlation with light microscopy, 0.98).
Hyperpigmented lesions gave rise to high signal, attenuating deeper
signal; hypopigmented lesions had the opposite effect on deeper signal.
conclusions. The inner band is not RNFL specific, partly consisting of a
surface-related signal. The location, not thickness, of the outer band
corresponds to RPE melanin. Given the additional effect of polarization
settings, precise OCT measurement of specific retinal layers is
This PDF is available to Subscribers Only