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Tristan Bourcier, Patricia Forgez, Vincent Borderie, Sarah Scheer, William Rostène, Laurent Laroche; Regulation of Human Corneal Epithelial Cell Proliferation and Apoptosis by Dexamethasone. Invest. Ophthalmol. Vis. Sci. 2000;41(13):4133-4141.
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purpose. To investigate whether human corneal epithelial cells express the
glucocorticoid receptor (GR) and to assess the influence of
dexamethasone (DEX) on these cells.
methods. Human corneal epithelial cells were cultured in medium supplemented
with various concentrations of DEX (ranging from
10− 10 to 10− 4 M).
Cell proliferation was analyzed by
inner salt (MTS) assay at 2, 4, and 6 days of culture. Apoptosis
was studied by nucleus labeling using a fluorescent dye and
immunostaining by APO 2.7 at 6 days of culture. GR mRNA was detected in
corneal epithelium and cultured corneal epithelial cells by means of
reverse transcription–polymerase chain reaction (RT-PCR).
Immunocytochemical staining of the epithelial cells was performed with
a monoclonal anti-human GR.
results. RT-PCR and immunocytochemistry showed the expression of GR (mRNA and
protein) in corneal epithelial cells. DEX significantly increased
corneal epithelial cell proliferation with concentrations ranging from
10− 10 to 10− 6 M, with
a maximum effect at 10− 7 M
(P < 0.005). However, DEX also induced apoptosis
of cultured corneal epithelial cells at any concentration used.
conclusions. These results indicate that human corneal epithelial cells express the
GR and proliferate in response to DEX stimulation which also induces
corneal epithelial cell apoptosis.
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