In situ hybridization of mouse eye cryosections using
digoxigenin-labeled cRNA probes. (
A) cGK I antisense probe,
retina; (
B) cGK I sense probe, retina; (
C) cGK II
antisense probe, retina; (
D) cGK II sense probe, retina;
(
E) cGK I antisense probe, ciliary processes; (
F)
cGK II antisense probe, ciliary processes; (
G) cGK I
antisense probe, lens; (
H) cGK II antisense probe, lens;
(
I) cGK I antisense probe, cornea; and (
J) cGK II
antisense probe, cornea. As with (
B) and (
D),
matched cryosections hybridized with either the cGK I or II sense
probes produced essentially no reaction product in all eye regions
examined (data not shown). However, varying amounts of nonspecific
deposition of color product were observed in the corneal stroma (see
I and
J). This artifact was also inconsistently
seen in cryosections treated with only the AP-conjugated,
anti-digoxigenin secondary antibody. Comparisons of relative levels of
cGK I and II in ocular tissues are presented in
Table 1 , columns B and
C. Abbreviations, see
Figure 1 .