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Huiming Chen, Jharna Ray, Virginia Scarpino, Gregory M. Acland, Gustavo D. Aguirre, Robert E. Anderson; Synthesis and Release of Docosahexaenoic Acid by the RPE Cells of prcd-Affected Dogs. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2418-2422.
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purpose. Dogs affected with progressive rod-cone degeneration (prcd)
have reduced levels of docosahexaenoic acid (DHA, 22:6n-3) in their
plasma and rod photoreceptor outer segments (ROS). Dietary
supplementation of DHA has failed to increase the ROS DHA levels to
that of unaffected control dogs. The present study was undertaken to
test the hypothesis that prcd-affected dogs have a
reduced capacity for the synthesis and/or release of DHA in retinal
pigment epithelial (RPE) cells.
methods. RPE cells (first passage cultures) from prcd-affected
and normal dogs were incubated with [3H]eicosapentaenoic
acid (EPA, 20:5n-3) for 24 and 72 hours. After incubation, the
radiolabeled fatty acids in the cells and media were analyzed.
results. DHA and all its metabolic intermediates were detected in RPE cells from prcd-affected and normal dogs. No significant difference
was found in the amount of products (including DHA) synthesized between
normal and affected RPE cells at either time point. In the culture
media, RPE cells from prcd-affected dogs released
significantly more DHA than cells from normal dogs after 72-hour
incubation, but not after 24-hour incubation.
conclusions. RPE cells from prcd-affected dogs can synthesize
and release DHA at least as efficiently as cells from normal dogs.
Therefore, synthesis of DHA from its precursor and its release from RPE
cells does not appear to contribute to the reduction in ROS DHA levels
found in prcd-affected animals.
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