Purchase this article with an account.
Atsushi Mizota, Emiko Adachi-Usami; Effect of Body Temperature on Electroretinogram of Mice. Invest. Ophthalmol. Vis. Sci. 2002;43(12):3754-3757.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To investigate the ERG alterations induced by changes in body temperature in mice.
methods. Three-week-old BALB/c mice were used. Rectal temperature was measured with a digital thermometer and taken as the body temperature. In experiment 1, the body temperature was kept at 33°C, and the ERGs elicited by a constant stimulus intensity were recorded every 5 minutes. In experiment 2, the body temperature was lowered in five steps from 38°C to 33°C, 28°C, 23°C, and 18°C. At each body temperature, ERGs elicited by different stimulus intensities were recorded.
results. In experiment 1, the mean amplitudes and implicit times of both the a- and b-waves did not change significantly. In experiment 2, the amplitude of both the a- and b-waves decreased significantly with a decrease in body temperature, and the implicit times of the a- and b-waves were prolonged with a decrease in body temperature.
conclusions. Body temperature greatly affects the amplitude and timing of the ERG. Great care must be taken to maintain as normal a body temperature as possible when using the ERG to evaluate the retina, especially in small animals such as mice.
This PDF is available to Subscribers Only