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John C. Merriam, Stefan Löfgren, Ralph Michael, Per Söderberg, James Dillon, Lei Zheng, Marcelo Ayala; An Action Spectrum for UV-B Radiation and the Rat Lens. Invest. Ophthalmol. Vis. Sci. 2000;41(9):2642-2647.
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purpose. To determine an action spectrum for UV-B radiation and the rat lens and
to show the effect of the atmosphere and the cornea on the action
methods. One eye of young female rats was exposed to 5-nm bandwidths of UV-B
radiation (290, 295, 300, 305, 310, and 315 nm). Light scattering of
exposed and nonexposed lenses was measured 1 week after irradiation. A
quadratic polynomial was fit to the dose–response curve for each wave
band. The dose at each wave band that produced a level of light
scattering greater than 95% of the nonexposed lenses was defined as
the maximum acceptable dose (MAD). Transmittance of the rat cornea was
measured with a fiberoptic spectrophotometer. The times to be exposed
to the MAD in Stockholm (59.3° N) and La Palma (28° N) were
results. Significant light scattering was detected after UV-B at 295, 300, 305,
310, and 315 nm. The lens was most sensitive to UV-B at 300 nm.
Correcting for corneal transmittance showed that the rat lens is at
least as sensitive to UV radiation at 295 nm as at 300 nm. The times to
be exposed to the MAD at each wave band were greater in Stockholm than
in La Palma, and in both locations the theoretical time to be exposed
to the MAD was least at 305 nm.
conclusions. After correcting for corneal transmittance, the biological sensitivity
of the rat lens to UV-B is at least as great at 295 nm as at 300 nm.
After correcting for transmittance by the atmosphere, UV-B at 305 nm is
the most likely wave band to injure the rat lens in both Stockholm and
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