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Zhong Dong, Moshira Ghabrial, Malkhan Katar, Rafael Fridman, Richard S. Berk; Membrane-Type Matrix Metalloproteinases in Mice Intracorneally Infected with Pseudomonas aeruginosa. Invest. Ophthalmol. Vis. Sci. 2000;41(13):4189-4194.
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purpose. To establish the presence of membrane-type matrix metalloproteinases
(MT-MMPs) in the cornea and their expression in naive and immunized
mice intracorneally infected with Pseudomonas
methods. Naive (unimmunized) and immunized C57BL/6J mice were infected with P. aeruginosa, and gene expression of MT-MMPs were
detected by RT-PCR. Immunoblot analysis and immunostaining were also
used to characterize the MT-MMP response in both sets of animals.
results. Expression of MT1-MMP, MT2-MMP, and MT3-MMP (MMP 14, 15, and 16) was
detected by RT-PCR and immunoblot analysis. Of the three MT-MMPs
detected, MT1-MMP exhibited the greatest expression at protein levels.
In general, a bell-shaped curve was obtained for each of the MT-MMPs in
naive mice, but all of them showed much less expression in the
immunized mice. MT1-MMP was localized in the epithelial tissue of the
cornea, whereas MT2-MMP and MT3-MMP were mainly found in the interface
between the epithelium and substantia propria.
conclusions. MT1-MMP was detected and expressed to a greater extent in naive mice
than MT2-MMP and MT3-MMP. Peak expression of all three MT-MMPs showed a
good correlation with the overall inflammatory response.
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