Purchase this article with an account.
I. Michael Wormstone, Shigeo Tamiya, Julia M. Marcantonio, John R. Reddan; Hepatocyte Growth Factor Function and c-Met Expression in Human Lens Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2000;41(13):4216-4222.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. Hepatocyte growth factor (HGF) and its receptor c-met perform a
multitude of functions. However, despite the significant degree of
study of HGF and c-met in numerous tissues and cell types, relatively
few investigations have been performed on the lens. In the current
study, therefore, the role of HGF and the receptor c-met in human lens
epithelial cells was investigated.
methods. Anterior epithelium and capsular bags were prepared from human donor
eyes and maintained in Eagle’s minimum essential medium (EMEM)
in a 5% CO2 atmosphere at 35°C. In addition, the human
lens cell line FHL124, was routinely cultured and seeded onto glass
coverslips (c-met immunodetection), 12-well plates (DNA and protein
synthesis), and tissue culture dishes (migration). c-Met was detected
by immunocytochemistry and fluorescence-activated cell scanning (FACS).
HGF was measured using enzyme-linked immunosorbent assay (ELISA)
techniques. Proliferation and protein synthesis were determined by[ 3H]thymidine and 35S-methionine
incorporation into DNA and proteins, respectively. Migration was
assessed using a scratch-wound assay and time-lapse video microscopy.
results. HGF was detected at all stages of culture of capsular bags in
protein-free medium. Moreover, c-met was present on the native
epithelium and after mechanical trauma was seen to be upregulated.
Immunolocalization and FACS analysis demonstrated c-met expression on
FHL124 cells throughout the whole population. Furthermore, FACS
analysis showed that serum-maintained cells sustained a higher level of
receptor expression relative to serum-deprived cells. Additionally, HGF
was found to stimulate proliferation, protein synthesis, and migratory
conclusions. c-Met receptors are expressed in native epithelium, capsular bag
cultures, and FHL124 cells. Receptor is distributed across the entire
cell population; however, this expression is environmentally and
mechanically sensitive. HGF is also present in capsular bags at all
stages of culture. In addition, HGF can stimulate migration,
proliferation, and protein synthesis. It therefore appears that a
multifunctional autocrine loop involving HGF and c-met is in place and
could be important in the development of posterior capsule
This PDF is available to Subscribers Only