Purchase this article with an account.
Kazuhiko Yoshida, Yinling Hu, Michael Karin; IκB Kinase α Is Essential for Development of the Mammalian Cornea and Conjunctiva. Invest. Ophthalmol. Vis. Sci. 2000;41(12):3665-3669. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To determine the requirement of IκB kinase α (Ikkα)
for differentiation of the mammalian cornea and conjunctiva.
methods. Newborn mice or surgically removed embryonic day (E)18 to E19 fetuses
of wild-type and Ikkα− /− mice
were analyzed by light microscopy and electron microscopy or
immunocytochemistry using anti-keratin (K)12, K4, K5, IκB, or nuclear
factor (NF)-κB (p50) antibody.
results. In the Ikkα− /− eyes, the epithelium of the cornea and the conjunctiva consisted of
poorly differentiated cells with round nuclei. K5 was much stronger in
the conjunctiva of the Ikkα− /− mice. Expression of K12 in the cornea and K4 in the conjunctiva was
impaired in the Ikkα− /− mice. IκB expression was low in epithelium of the cornea and
conjunctiva of the wild type mice but was very strong in that of the Ikkα− /− mice. During normal development of the conjunctiva, nuclear
localization of p50 was seen in areas where basal undifferentiated
cells give rise to differentiated cell types, marked by expression of
cK4. However, in the Ikkα− /− tissues, no nuclear p50 staining was detected.
conclusions. IKKα is specifically required for formation of cornea and
conjunctiva. This function may be exerted through an effect on NF-κB
This PDF is available to Subscribers Only