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Masaru Inatani, Hidenobu Tanihara, Atsuhiko Oohira, Yasumasa Otori, Akihiro Nishida, Megumi Honjo, Noriaki Kido, Yoshihito Honda; Neuroglycan C, a Neural Tissue–Specific Transmembrane Chondroitin Sulfate Proteoglycan, in Retinal Neural Network Formation. Invest. Ophthalmol. Vis. Sci. 2000;41(13):4338-4346.
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© ARVO (1962-2015); The Authors (2016-present)
purpose. Neuroglycan C (NGC) is a transmembrane chondroitin sulfate proteoglycan
present exclusively in central nervous system tissues. In the current
study the expression pattern and characterization of NGC during the
development of the retina were investigated.
methods. Expressional changes of NGC mRNAs during rat retinal development were
examined by semiquantitative reverse transcription–polymerase chain
reaction (RT-PCR). The localization and characterization of NGC core
proteins were investigated by immunoblot analysis and
immunohistochemistry using an anti-NGC antibody.
results. Immunohistochemical analysis revealed that NGC was highly expressed in
the nerve fiber layer (NFL) and inner plexiform layer (IPL) in rat
postnatal developing retina. At embryonal stages, NGC
immunoreactivities were faint. In contrast, at postnatal developmental
stages (approximately postnatal day [P]7), intense immunoreactivity
was observed in the NFL and IPL, where active dendrite branching was
observed, and conventional synapses began to be formed. As retinal
layer differentiation proceeded (from P14 to P42), immunoreactivities
in the inner retinal layers gradually became fainter. Immunoblot and
semiquantitative RT-PCR analyses showed that the peak level of NGC
expression occurred on approximately P7 and P14. Glycosylation of the
NGC core protein changed as the retinal layers matured. In
immunoelectron microscopic analysis, NGC immunoreactivity was located
on the axonal membranes of neuronal cells in the postnatal retina,
whereas immunoreactivity was reduced on membranes at the adult stage.
In retinal ganglion cells in vitro, NGC was highly localized in their
spiny budding neurites.
conclusions. The results show spatiotemporal expression patterns of NGC, and suggest
that it plays a role in the formation of neural networks in retinal
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