Purchase this article with an account.
Ulrich Welge–Lüßen, Christian Albrecht May, Michael Eichhorn, Hans Bloemendal, Elke Lütjen–Drecoll; αB-Crystallin in the Trabecular Meshwork Is Inducible by Transforming Growth Factor-β. Invest. Ophthalmol. Vis. Sci. 1999;40(10):2235-2241.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. Because in glaucomatous eyes transforming growth factor-β (TGF-β)
and αB-crystallin are increased in the anterior eye segment, the
effect of TGF-β1 and TGF-β2 on the expression of αB-crystallin
and its corresponding mRNA was studied in human trabecular meshwork
methods. Monolayer cultures of “cribriform” and “corneoscleral” TM cells
of 5 human donors (12–73 years of age) were treated with either 1.0
ng/ml TGF-β1, TGF-β2, or 5 × 10−7 dexamethasone
(DEX) for 12 to 96 hours. Induction of αB-crystallin and the related
mRNA was investigated by western and northern blot analyses. For
comparison, human foreskin fibroblasts (HFF) and NIH 3T3 cells were
treated in the same way as the TM cells.
results. An increase of αB-crystallin mRNA was observed after treatment of TM
cells with TGF-β1 and TGF-β2, whereas DEX had no effect. In the
cribriform TM cells with a high basal level, the enhancement ranged
between 2 and 3 times; whereas in the corneoscleral TM cellsα
B-crystallin mRNA increased between 5 and 6 times. Using western
blot analysis, the increase of αB-crystallin expression in the
cribriform TM cells was only small compared with the significant
increase in the corneoscleral TM cells. Treatment of HFF and NIH 3T3
cells with TGF-β did not induce αB-crystallin mRNA.
conclusions. This is the first time to show that αB-crystallin is not only induced
by stress factors but also by TGF-β in TM cell cultures. The
difference in induction of mRNA and protein seems to be dependent onα
B-crystallin concentration before
This PDF is available to Subscribers Only