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Junjie Yang, Gülgün Tezel, Rajkumar V. Patil, Carmelo Romano, Martin B. Wax; Serum Autoantibody against Glutathione S-Transferase in Patients with Glaucoma. Invest. Ophthalmol. Vis. Sci. 2001;42(6):1273-1276.
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purpose. To identify retinal proteins that are the targets of serum
autoantibodies in patients with glaucoma.
methods. To identify retinal antigens that are recognized by the sera of
patients with glaucoma, immunoreactive bands were separated, by using
two-dimensional gel electrophoresis of the bovine retinal soluble
fraction. A 29-kDa band was then selected for further analysis. Tryptic
peptides of the 29-kDa band were analyzed using electrospray mass
spectrometry to identify the protein. After protein identification,
immunoreactivity against this newly identified protein was studied by
Western blot analysis using sera from 65 patients with glaucoma (25
with primary open-angle glaucoma [POAG]; 40 with normal-pressure
glaucoma [NPG]) and 25 age-matched healthy subjects. In addition,
serum antibody titers were compared in these groups, by using a
specific enzyme-linked immunosorbent assay (ELISA).
results. The 29-kDa band was identified as glutathione S-transferase (GST). Western blot analysis revealed that
serum antibodies against GST antigen were recognized in 34 (52%) of 65
patients with glaucoma (22 of NPG and 12 of POAG) and 5 (20%) of 25
age-matched control subjects (χ2 test, P < 0.05). By ELISA, it was also found that
patients with glaucoma had higher titers of anti-GST antibody, compared
with the control group (Mann–Whitney test; NPG versus control, P = 0.013; POAG versus control, P = 0.0006).
conclusions. These findings indicate that GST is one of the retinal antigens
targeted by the serum antibodies detected in some patients with
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