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Tatiana Ulyanova, Agoston Szél, R. Krishnan Kutty, Barbara Wiggert, A. Romeo Caffé, Gerald J. Chader, Theo van Veen; Oxidative Stress Induces Heme Oxygenase-1 Immunoreactivity in Müller Cells of Mouse Retina in Organ Culture. Invest. Ophthalmol. Vis. Sci. 2001;42(6):1370-1374.
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purpose. Heme oxygenase (HO)-1 immunoreactivity (IR) was examined in normal
untreated retina and in retinal explants after in vitro treatment with
methods. Enucleated eyes from young adult C3H mice were immediately fixed and
cryosectioned and the retina sections processed for immunocytochemistry
with antibodies against HO-1 and glial fibrillary acidic protein
(GFAP). From other eyes retinas were isolated and maintained in organ
culture, either untreated for 4 days maximum or for 21 hours during
which the explants were treated the first 3 hours with selected doses
of sodium arsenate or hydrogen peroxide. Thereafter, the explants were
processed identically with the normal tissue.
results. In the normal retina, HO-1 and GFAP IR was very low. The culturing
itself resulted in an increase in both HO-1 and GFAP immunolabeling in
Müller cells of explanted retinas. Both sodium arsenate and
hydrogen peroxide further induced strong HO-1 IR in Müller cells
but not in other retinal cells. In contrast to HO-1, GFAP staining in
Müller cells was not altered as a result of treatment, either by
sodium arsenate or hydrogen peroxide at any concentration used.
conclusions. The results show for the first time that HO-1 can be induced in the
retina in vitro by conditions of oxidative stress and that enzyme
expression is confined exclusively to Müller
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