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Shin–ichiro Hirai, Naruhiro Ishida, Katsuhiko Watanabe, Shiro Mita; Leakage of Aquaporin 5 in the Tear of Dacryoadenitis Mice. Invest. Ophthalmol. Vis. Sci. 2000;41(9):2432-2437.
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purpose. The objective of this study was to investigate whether leakage of
aquaporin 5 (AQP5) in tear is associated with damage of lacrimal glands
(LGs) in dacryoadenitis models.
methods. Female MRL/lpr (24-week-old), male NOD/Shi Jci (5-, 8-, and
10-week-old), female NFS/s-TX (10-week-old), and lipopolysaccharide
(LPS)-induced dacryoadenitis model mice were used. Tear fluid was
collected by a cotton thread. Tear proteins in the thread were
dissolved in sodium dodecyl sulfate buffer, and AQP5 proteins were
analyzed by the Western blot technique using anti-AQP5 antibody. LGs
were prepared for hematoxylin and eosin staining or immunostaining of
results. In MRL/lpr, NFS/s-TX, 8- and 10-week-old NOD/Shi Jci mice, AQP5 protein
was detected in the tear by Western blot analysis. Inflammatory
lymphocyte infiltrations were observed in LGs of these dacryoadenitis
model mice. In contrast, AQP5 leakage and damage of LG were not
observed in normal mice. In 5-week-old NOD/Shi Jci mice, infiltration
was not seen in LG, and AQP5 leakage was not detected in the tear. In
LPS-induced dacryoadenitis model mice, either tissue destruction with
inflammation in LG or AQP5 leakage in the tear was observed. AQP5 in
the tear and tissue inflammation in LGs was not found in control mice.
These results indicate that AQP5 is leaked in tears when LGs are
damaged by dacryoadenitis.
conclusions. Leakage of AQP5 in the tear was found to be related to LG damage. This
finding suggests that detection of AQP5 in tear is useful for specific
diagnosis of LG disorders with tissue
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