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Yasushi Ikuno, Fee-Lai Leong, Andrius Kazlauskas; PI3K and PLCγ Play a Central Role in Experimental PVR. Invest. Ophthalmol. Vis. Sci. 2002;43(2):483-489.
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purpose. It has been reported that the platelet-derived growth factor (PDGF)-α
receptor (αPDGFR) is required for experimental proliferative
vitreoretinopathy (PVR) in rabbits. This study investigated
which of the signaling enzymes downstream of the αPDGFR participate
methods. A panel of cell lines that expressed αPDGFR signaling mutants were
made and characterized. These cell lines were used in a rabbit model of
PVR and in an in vitro collagen type I contraction assay.
results. Phosphoinositide 3-kinase (PI3K) and, to a lesser extent, phospholipase
C (PLC)-γ were the signaling enzymes required for the αPDGFR to
mediate PVR. Furthermore, the cells lines that were the most effective
at inducing PVR displayed the most potent activity in the in vitro
conclusions. PI3K and PLCγ are necessary downstream effectors of the αPDGFR in
experimental PVR. Consequently, these two signaling enzymes are
required for one or more of the cellular responses (chemotaxis,
proliferation, extracellular matrix production, contraction) that
contribute to PVR.
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